Isolation of Neospora caninum genes detected during a chronic murine infection

Atkinson, R.A., Ryce, C., Miller, C.M.D., Balu, S., Harper, P.A.W., and Ellis, J.T. (2001) Isolation of Neospora caninum genes detected during a chronic murine infection. International Journal for Parasitology, 31 (1). pp. 67-71.

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Abstract

In order to isolate genes coding for antigens of Neospora caninum which are recognised by the host immune system during a chronic murine infection, a cDNA library was immunoscreened with pooled sera from mice which survived three independent infections by N. caninum. Two new genes from N. caninum were isolated and expressed in Escherichia coli. The genes identified include one homologous to GRA1 of Toxoplasma gondii, plus another (NCP20) previously unknown in any taxon. Both genes encode small polypeptides which induced an IgG response in the mouse and were also recognised by IgG from a cow chronically infected with N. caninum. These results are consistent with the hypothesis that the polypeptides encoded by these genes are a target for the host immune system during chronic infections of N. caninum.

Item ID: 34603
Item Type: Article (Research - C1)
ISSN: 1879-0135
Keywords: Neospora caninum; western blotting; GRA-1; expression library;immunoscreening
Funders: Australian Research Council (ARC), University of Technology, Sydney
Date Deposited: 26 Aug 2014 04:19
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070705 Veterinary Immunology @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 50%
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