Sperm transport, size of the seminal plug and the timing of ovulation after natural mating in the female tammar wallaby Macropus eugenii

Paris, Damien, Taggart, David. A., Paris, Monica C.J., Temple-Smith, Peter D., and Renfree, Marilyn B. (2004) Sperm transport, size of the seminal plug and the timing of ovulation after natural mating in the female tammar wallaby Macropus eugenii. Reproduction, Fertility and Development, 16. pp. 811-822.

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Abstract

The distribution of spermatozoa and seminal plug in the reproductive tract and the timing of ovulation were examined at various times in a naturally mated monovular macropodid marsupial, namely the tammar wallaby (Macropus eugenii). After the first post partum (p.p.) mating, 28 females were isolated and their reproductive tracts dissected at 0.5, 6, 18, 36 and 40 h post coitum (p.c.). Each tract was ligated into 13 major anatomical sections and spermatozoa and eggs were recovered by flushing. Mating was possibly delayed by handling and occurred 21.7 ± 2.5 h p.p. in these animals. Copulation lasted 7.8 ± 0.7 min. Within 0.5 h after a single mating, the tract contained 25.8 ± 10.2 × 106 spermatozoa and 21.6 ± 8.8 g of seminal plug, 96% and 70% of which was lost within 6 h p.c. respectively. Spermatozoa reached the uterus, isthmus and ampulla of the oviduct on the side of the developing follicle within 0.5, 6 and 18 h p.c., respectively, and a uterine population of 26.1 ± 12.103 spermatozoa was maintained for over 40 h. Sperm numbers were reduced at the cervix (up to 57-fold) and uterotubule junction (eight-fold) and only one in approximately 7500 ejaculated spermatozoa (3.4 ± 0.9 × 103) reached the oviduct on the follicle side. Differential transport of spermatozoa was not observed. Although the numbers of spermatozoa were reduced in the parturient uterus, they were highly variable and were not significantly different to those in the non-parturient uterus. Ovulation and recovery of sperm-covered eggs from the isthmus occurred 36–41 h p.c. (49–72 h p.p.). In contrast with the polyovular dasyurid and didelphid marsupials, the tammar wallaby ejaculates large numbers of spermatozoa, but transport is relatively inefficient and sperm storage in the tract before ovulation is limited.

Item ID: 34530
Item Type: Article (Research - C1)
ISSN: 1448-5990
Keywords: marsupial, copulation, copulatory plug, oestrus, sperm reservoir
Funders: Australian Research Council (ARC), University of Melbourne
Projects and Grants: ARC SPIRT grant No. C09930004
Date Deposited: 27 Aug 2014 03:52
FoR Codes: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060803 Animal Developmental and Reproductive Biology @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 80%
83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830305 Game Livestock (e.g. Kangaroos, Wallabies, Camels, Buffaloes, Possums) @ 20%
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