Carotenoid genes transcriptional regulation for astaxanthin accumulation in fresh water unicellular alga Haematococcus pluvialis by gibberellin A3 (GA3)

Gao, Zhengquan, Meng, Chunxiao, Gao, Hongzheng, Li, Yan, Zhang, Xiaowen, Xu, Dong, Zhou, Shitan, Liu, Banghui, Su, Yuanfeng, and Ye, Naihao (2013) Carotenoid genes transcriptional regulation for astaxanthin accumulation in fresh water unicellular alga Haematococcus pluvialis by gibberellin A3 (GA3). Indian Journal of Biochemistry and Biophysics, 50 (6). pp. 548-553.

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Abstract

The fresh water unicellular alga Haematococcus pluvialis is a promising natural source of astaxanthin. The present study investigated the transcriptional expression of carotenoid genes for astaxanthin accumulation in H. pluvialis using real-time fluorescence quantitative PCR (qRT-PCR). With treatments of 20 and 40 mg/L of gibberllin A(3) (GA(3)), five genes ipi-1, ipi-2, psy, pds and bkt2 were up-regulated with different expression profiles. GA20 (20 mg/L of GA(3)) treatment had a greater effect on transcriptional expression of bkt2 than on ipi-1 ipi-2, psy and pds (>4-fold up-regulation). However, GA40 (40 mg/L of GA,) induced more transcriptional expression of ipi-2, pry and bkt2 than both ipi-1 and pds. The expression of lyc, crtR-B and crtO for astaxanthin biosynthesis was not affected by GA(3) in H. piuvialis. In the presence of GA(3), astaxanthin biosynthesis genes of ipi-1, pds and bkt2 were up-regulated at transcriptional level, pry at post-transcriptional level, whereas ipi-2 was up-regulated at both levels. The study could potentially lead to a scale application of exogenous GA(3) in astaxanthin production with H. pluvialis just like GAs perform in increasing crops production and it would provide new insight about the multifunctional roles of carotenogenesis in response to GA(3).

Item ID: 32884
Item Type: Article (Research - C1)
ISSN: 0975-0959
Keywords: Haematococcus pluvialis, astaxanthin, gibberellin A3 (GA(3)), carotenoid genes, real-time fluorescence quantitative PCR
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Funders: National Natural Science Foundation of China, National Natural Science Foundation of Shandong Province, Open Funds of the State Key Laboratory of Agricultural Microbiology, Shandong University of Technology, James Cook University (JCU)
Projects and Grants: National Natural Science Foundation of China (41106124, 31170279), National Natural Science Foundation of Shandong Province (ZR2011DM006, ZR2011CQ010), Open Funds of the State Key Laboratory of Agricultural Microbiology (AMLKF201003), Shandong University of Technology (4072-110045), JCU (20732)
Date Deposited: 30 Apr 2014 09:55
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 50%
06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060701 Phycology (incl Marine Grasses) @ 50%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8299 Other Plant Production and Plant Primary Products > 829999 Plant Production and Plant Primary Products not elsewhere classified @ 100%
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