Carbonic anhydrase inhibitors. Inhibition studies with anions and sulfonamides of a new cytosolic enzyme from the scleractinian coral Stylophora pistillata

Bertucci, Anthony, Innocenti, Alessio, Scozzafava, Andrea, Tambutté, Sylvie, Zoccola, Didier, and Supuran, Claudiu T. (2011) Carbonic anhydrase inhibitors. Inhibition studies with anions and sulfonamides of a new cytosolic enzyme from the scleractinian coral Stylophora pistillata. Bioorganic and Medicinal Chemistry Letters, 21 (2). pp. 710-714.

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Abstract

The catalytic activity and the inhibition of a new coral carbonic anhydrase (CA, EC 4.2.1.1), from the scleractinian coral Stylophora pistillata, STPCA-2, has been investigated. STPCA-2 has high catalytic activity for the physiological reaction being less sensitive to anion and sulfonamide inhibitors compared to STPCA, a coral enzyme previously described. The best STPCA-2 anion inhibitors were sulfamide, sulfamic acid, phenylboronic acid, and phenylarsonic acid (KIs of 5.7–67.2 μM) whereas the best sulfonamide inhibitors were acetazolamide and dichlorophenamide (KIs of 74–79 nM). Because this discriminatory effect between these two coral CAs, sulfonamides may be useful to better understand the physiological role of STPCA and STPCA-2 in corals and biomineralization processes.

Item ID: 31636
Item Type: Article (Research - C1)
ISSN: 1464-3405
Keywords: carbonic anhydrase; coral; Stylophora pistillata; anion inhibitor; sulfonamide; carbon fixation
Funders: European Union Sixth Framework Program (EU FP6), Fund for Investment in Basic Research (FIRB), Government of the Principality of Monaco
Projects and Grants: EU FP6 DeZnIT project, FIRB MIUR/FIRB RBNE03PX83_001
Date Deposited: 16 Oct 2014 02:21
FoR Codes: 06 BIOLOGICAL SCIENCES > 0606 Physiology > 060604 Comparative Physiology @ 50%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060107 Enzymes @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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