Differential expression and glycosylation of proteins in the rat ileal epithelium in response to Echinostoma caproni infection

Muñoz-Antoli, Carla, Cortés, Alba, Sotillo, Javier, Fried, Bernard, Esteban, J. Guillermo, and Toledo, Rafael (2014) Differential expression and glycosylation of proteins in the rat ileal epithelium in response to Echinostoma caproni infection. Journal of Proteomics, 101. pp. 169-178.

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Abstract

Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode that has been used as experimental model to investigate the factors determining the expulsion of intestinal helminths. We analyze the changes in the protein expression and glycosylation induced by E. caproni in Wistar rat, a host of low compatibility in which the parasites are rapidly rejected. To determine the changes in protein expression, two-dimensional difference gel electrophoresis was employed using protein extracts from the intestine of naïve and infected rats. The patterns of glycosylation were analyzed by lectin blotting. Those spots showing differential expression or glycosylation were analyzed by mass spectrometry. A total of 33 protein spots differentially expressed were identified (26 were found to be over-expressed and 7 down-regulated). Moreover, E. caproni induced changes in the glycosylation status of 8 proteins that were successfully identified. Most of these proteins were related to the cytoskeleton and the maintenance of the functional integrity of the ileal epithelium. This suggests that the regeneration of the intestinal tissue is a major effector mechanism responsible for the early expulsion of this helminth. Furthermore, several proteins involved in the energy metabolism were also altered in the ileum of rats as a consequence of the E. caproni infection.

Item ID: 31475
Item Type: Article (Research - C1)
ISSN: 1876-7737
Keywords: Echinostoma caproni; helminth; rat intestine; protein expression; protein glycosylation; worm expulsion
Funders: Conselleria d’Educació , Generalitat Valenciana, Universitat de Valencia
Projects and Grants: Conselleria d’Educació , Generalitat Valenciana PROMETEO/2009/081, Universitat de Valencia INV-AE13-136845
Date Deposited: 13 Jun 2014 04:54
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) @ 40%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 20%
06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060307 Host-Parasite Interactions @ 40%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 25%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 25%
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