Investigation of an idiopathic lesion in redclaw crayfish Cherax quadricarinatus using suppression subtractive hybridization

Rusaini, , Ariel, Ellen, Burgess, Graham W., and Owens, Leigh (2013) Investigation of an idiopathic lesion in redclaw crayfish Cherax quadricarinatus using suppression subtractive hybridization. Journal of Virology & Microbiology, 2013. pp. 1-15.

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Abstract

Lesions indicative of putative parvovirus infection were detected in a population of freshwater redclaw crayfish (Cherax quadricarinatus) displaying hypertrophied nuclei, while these changes were undetected in another population. These changes were characterised by hypertrophic nuclei with marginated chromatin but without Cowdry type A (CA) intranuclear inclusion bodies. Suppression subtractive hybridization (SSH) was performed to generate a cDNA forward library between the two populations of crayfish. A total of 323 sequences were analysed from SSH libraries. These sequences were grouped into 76 consensus sequences and clustered into 8 categories based on sequence homology from the NCBI GenBank database. Even though histopathological observations consistently revealed lesions presumptive of putative parvovirus in C. quadricarinatus as previously reported, the molecular method failed to confirm a viral aetiology. Despite the absence of viral gene detection and therefore a conclusion of an idiopathic aetiology, the health status of these two populations of redclaw crayfish was different, resulting in differentially expressed immune-related genes in the two populations, with some genes being up-regulated in the hypertrophied nuclei population. Furthermore, the absence of parvovirus from the SSH libraries indicates that perhaps the viral messenger RNA was in too low a concentration to be detected or does not have any poly(A) tail which the SSH methodology needs to function correctly.

Item ID: 31294
Item Type: Article (Refereed Research - C1)
Keywords: redclaw crayfish, parvovirus, suppression subtractive hybridization, idiopathic aetiology
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© 2013 Rusaini, Ellen Ariel, Graham W. Burgess and Leigh Owens. Distributed under Creative Commons CC-BY 3.0

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ISSN: 2326-7011
Funders: James Cook University (JCU), Australian Agency for International Development (AusAID)
Projects and Grants: JCU Graduate Research Scheme (GRS) Grant 2011
Date Deposited: 28 Feb 2014 06:18
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070404 Fish Pests and Diseases @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 60%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 40%
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