Aldehyded dextran and ε-poly(l-lysine) hydrogel as nonviral gene carrier

Togo, Yumiko , Takahashi, Katsu, Saito, Kazuyuki, Kiso, Honoka, Huang, Boyen, Tsukamoto, Hiroko, Hyon, Suong-Hyu, and Bessho, Kazuhisa (2013) Aldehyded dextran and ε-poly(l-lysine) hydrogel as nonviral gene carrier. Stem Cells International, 2013. pp. 1-5.

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Abstract

The expression term of the gene transfected in cells needs to belong enough inorder to make a gene therapy clinically effective. The controlled release of the transfected gene can be utilized. The new biodegradable hydrogel material created by 20 w/w% aldehyded dextran and 10 w/w% ε-poly(L-lysine) (ald-dex/PLL) was developed. We examined whether it could be as a nonviral carrier of the gene transfer. Methods. A plasmid (Lac-Z) was mixed with ald-dex/PLL. An in vitro study was performed to assess the expression of Lac-Z with X-gal stain after gene transfer into the cultured 293 cells and bone marrow cells. As a control group, PLL was used as a cationic polymer. Results. We confirmed that the transfection efficiency of the ald-dex/PLL had a higher transfection efficiency than PLL in 293 cells (plasmid of 2 μg: ald-dex/PLL 1.1%, PLL 0.23%, plasmid of 16 μg: ald-dex/PLL 1.23%, PLL 0.48%). In bone marrow cells, we confirmed the expression of Lac-Z by changing the quantity of aldehyded dextran. In the groups using ald-dextran of the quantity of 1/4 and 1/12 of PLL, their transfection efficiency was 0.43% and 0.41%, respectively. Conclusions. This study suggested a potential of using ald-dex/PLL as a non-carrier for gene transfer.

Item ID: 29594
Item Type: Article (Refereed Research - C1)
Additional Information:

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

ISSN: 1687-9678
Date Deposited: 04 Oct 2013 00:06
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 20%
10 TECHNOLOGY > 1004 Medical Biotechnology > 100401 Gene and Molecular Therapy @ 20%
09 ENGINEERING > 0903 Biomedical Engineering > 090301 Biomaterials @ 60%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920110 Inherited Diseases (incl. Gene Therapy) @ 30%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified @ 70%
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