Aldehyded dextran and ε-poly(l-lysine) hydrogel as nonviral gene carrier
Togo, Yumiko , Takahashi, Katsu, Saito, Kazuyuki, Kiso, Honoka, Huang, Boyen, Tsukamoto, Hiroko, Hyon, Suong-Hyu, and Bessho, Kazuhisa (2013) Aldehyded dextran and ε-poly(l-lysine) hydrogel as nonviral gene carrier. Stem Cells International, 2013. 634379. pp. 1-5.
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Abstract
The expression term of the gene transfected in cells needs to belong enough inorder to make a gene therapy clinically effective. The controlled release of the transfected gene can be utilized. The new biodegradable hydrogel material created by 20 w/w% aldehyded dextran and 10 w/w% ε-poly(L-lysine) (ald-dex/PLL) was developed. We examined whether it could be as a nonviral carrier of the gene transfer. Methods. A plasmid (Lac-Z) was mixed with ald-dex/PLL. An in vitro study was performed to assess the expression of Lac-Z with X-gal stain after gene transfer into the cultured 293 cells and bone marrow cells. As a control group, PLL was used as a cationic polymer. Results. We confirmed that the transfection efficiency of the ald-dex/PLL had a higher transfection efficiency than PLL in 293 cells (plasmid of 2 μg: ald-dex/PLL 1.1%, PLL 0.23%, plasmid of 16 μg: ald-dex/PLL 1.23%, PLL 0.48%). In bone marrow cells, we confirmed the expression of Lac-Z by changing the quantity of aldehyded dextran. In the groups using ald-dextran of the quantity of 1/4 and 1/12 of PLL, their transfection efficiency was 0.43% and 0.41%, respectively. Conclusions. This study suggested a potential of using ald-dex/PLL as a non-carrier for gene transfer.
Item ID: | 29594 |
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Item Type: | Article (Research - C1) |
ISSN: | 1687-9678 |
Additional Information: | This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Date Deposited: | 04 Oct 2013 00:06 |
FoR Codes: | 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 20% 10 TECHNOLOGY > 1004 Medical Biotechnology > 100401 Gene and Molecular Therapy @ 20% 09 ENGINEERING > 0903 Biomedical Engineering > 090301 Biomaterials @ 60% |
SEO Codes: | 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920110 Inherited Diseases (incl. Gene Therapy) @ 30% 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified @ 70% |
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