Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon

Montanari, Stefano R., Gardner, Michael G., Hobbs, Jean-Paul A., Pratchett, Morgan S., Bay, Line K., and van Herwerden, Lynne (2013) Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon. Conservation Genetics Resources, 5 (3). pp. 783-786.

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DOI: 10.1007/s12686-013-9907-7

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Abstract

Twenty polymorphic microsatellite loci were developed via 454 sequencing for two hybridizing sister species of butterflyfish: the spot-band butterflyfish (Chaetodon punctatofasciatus) and peppered butterflyfish (Chaetodon guttatissimus), which are widely distributed in the Western Pacific and Indian Ocean, respectively. All loci were genotyped in samples collected from Christmas Island: C. guttatissimus (n = 25), C. punctatofasciatus (n = 17) and hybrids (n = 16). Mean alleles per locus (N a ) were: 9.05 for C. guttatissimus, 9.95 for C. punctatofasciatus and 9.45 for hybrids. Observed heterozygosity (H O ) ranged from 0.00 to 1.00 for C. guttatissimus; from 0.08 to 0.88 for C. punctatofasciatus; and from 0.19 to 0.94 for hybrids. Most loci conformed to Hardy–Weinberg expectations, were in linkage equilibrium, and did not contain null alleles. These markers will be useful for testing population genetic hypotheses including patterns of hybridization in this pair of butterflyfishes.


Item ID:	29513
Item Type:	Article (Research - C1)
ISSN:	1877-7260
Keywords:	allopatry, butterflyfish, Christmas Island, hybridization, Indo-Pacific, suture zone
Related URLs:	https://researchonline.jcu.edu.au/58946/
Additional Information:	A version of this publication was included as Chapter 2 of the following PhD thesis: Montanari, Stefano R. (2018) Causes and consequences of natural hybridisation among coral reef butterflyfishes (Chaetodon: Chaetodontidae). PhD thesis, James Cook University, which is available Open Access in ResearchOnline@JCU. Please see the Related URLs for access.
Funders:	ARC Centre of Excellence for Coral Reef Studies, Australian Institute of Marine Science (AIMS)
Research Data:	http://datadryad.org/resource/doi:10.5061/dryad.jd183
Date Deposited:	27 Nov 2013 00:25
FoR Codes:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes:	96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%
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