Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish

Siregar, Tuti Hartati, Elliman, Jennifer, and Owens, Leigh (2012) Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish. Squalen, 7 (2). pp. 51-58.

[img]
Preview
PDF (Published Version) - Published Version
Available under License Creative Commons Attribution.

Download (597kB)
View at Publisher Website: http://www.bbp4b.litbang.kkp.go.id/squal...
 
1192


Abstract

Previously designed endpoint PCR has been adapted for use with real time PCR to detect the presence of Salmonella typhimurium and Salmonella enteritidis in fish. Optimization of a standard curve in the presence of herring sperm DNA as background matrix indicated that the real time PCR highly efficient with the Pearson coefficient of determination (R2) value = 0.99937 and slope (M) value = -3.44. An enrichment method (overnight culture) significantly increased (p<0.05) the sensitivity of real time PCR. Comparison of real time PCR and the conventional isolation method based on biochemical tests has been conducted. In terms of their sensitivity, real time PCR and the conventional methods are not significantly different in the level of confidence 95%. Both real time PCR with enrichment method and conventional biochemical method can detect the presence of Salmonella spp. in spiked sample. However the direct extraction method was only detecting the presence of Salmonella in higher concentration. While the sensitivity both conventional and real time PCR are similar, the real time PCR has an advantage to detect the pathogen qualitatively and quantitatively depending on processing method.

Item ID: 28657
Item Type: Article (Research - C1)
ISSN: 2089-5690
Keywords: real time PCR, Salmonella contamination, fish
Additional Information:

This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This work is licensed under a Creative Commons Attribution 3.0 License.

Date Deposited: 14 Aug 2013 22:58
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 50%
86 MANUFACTURING > 8601 Processed Food Products and Beverages (excl. Dairy Products) > 860107 Processed Fish and Seafood Products @ 50%
Downloads: Total: 1192
Last 12 Months: 21
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page