Triploidy induction in Australian greenlip abalone Haliotis laevigata (Donovan) with cytochalasin B

Li , Yan, Li, Xiaoxu, and Qin, Jian G. (2007) Triploidy induction in Australian greenlip abalone Haliotis laevigata (Donovan) with cytochalasin B. Aquaculture Research, 38 (5). pp. 487-492.

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Abstract

Triploid induction in Australian greenlip abalone, Haliotis laevigata (Donovan), was conducted by blocking the formation of the second polar body using cytochalasin B (CB). Twenty minutes after fertilization, the zygotes of greenlip abalone were treated with four CB concentrations (0, 0.25, 0.5 and 0.75 mg L⁻¹) for 10, 15 and 20 min. The ploidy of resultant larvae was determined using flow cytometry at 72-h post fertilization. Our study showed that fertilization, hatching, survival and induced triploidy of abalone larvae were significantly affected by the CB concentration and treatment duration. The effective range of CB concentration for triploid induction on greenlip abalone was 0.5–0.75 mg L⁻¹ with an induction duration of 10–15 min. The results indicate that the most effective treatment combination for triploid induction in greenlip abalone is 0.5 mg CB L⁻¹ for 15 min starting at 20-min post fertilization.

Item ID: 28119
Item Type: Article (Research - C1)
ISSN: 1365-2109
Keywords: greenlip abalone, cytochalasin B, induced triploidy, flow cytometry
Date Deposited: 24 Jul 2013 00:21
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 50%
10 TECHNOLOGY > 1001 Agricultural Biotechnology > 100102 Agricultural Marine Biotechnology @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8398 Environmentally Sustainable Animal Production > 839899 Environmentally Sustainable Animal Production not elsewhere classified @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
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