Restriction fragment length analysis of the cytochrome b gene and muscle fatty acid composition differentiate the cryptic flatfish species Solea solea and Solea aegyptiaca

Boukouvala, Evridiki, Cariani, Alessia, Maes, Gregory E., Sevilla, Rafael G., Verrez-Bagnis, Veronique, Jerome, Marc, Guarniero, Ilaria, Monios, Georgios, Tinti, Fausto, Volckaert, Filip A.M., Bautista, Jose M., and Krey, Grigorios (2012) Restriction fragment length analysis of the cytochrome b gene and muscle fatty acid composition differentiate the cryptic flatfish species Solea solea and Solea aegyptiaca. Journal of Agricultural and Food Chemistry, 60 (32). pp. 7941-7948.

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Abstract

Overlapping external morphometric characters easily confound the flatfishes Solea aegyptiaca and Solea solea (Soleidae) in areas of the Mediterranean Sea where both species live in sympatry. This leads to uncertainties in the fisheries and marketing of the species, in addition to misinterpretations in biogeography and conservation studies. This paper describes a simple restriction fragment length-based diagnostic test that differentiates S. solea from S. aegyptiaca, as well as from other species of the Soleidae family. Furthermore, the two species living in sympatry in the Gulf of Kavala (North Aegean Sea, Greece) present significant qualitative differences in muscle fatty acid composition, a property that can also be used to distinguish the two cryptic species.

Item ID: 27687
Item Type: Article (Refereed Research - C1)
Keywords: Solea solea, Solea aegyptiaca, species diagnosis, cytochrome b gene, EcoR V restriction fragment length, white muscle fatty acid composition
ISSN: 1520-5118
Date Deposited: 18 Sep 2013 03:11
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 50%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) @ 30%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070405 Fish Physiology and Genetics @ 20%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8302 Fisheries - Wild Caught > 830204 Wild Caught Fin Fish (excl. Tuna) @ 50%
83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8399 Other Animal Production and Animal Primary Products > 839902 Fish Product Traceability and Quality Assurance @ 50%
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