Microsatellite marker development and putative SNP detection for a northward expanding damselfly species using next generation sequencing
Swaegers, J., Mergeay, J., Maes, G.E., Van Houdt, J.K.J., Larmuseau, M.H.D., and Stoks, R. (2012) Microsatellite marker development and putative SNP detection for a northward expanding damselfly species using next generation sequencing. Conservation Genetics Resources, 4 (4). pp. 1079-1084.
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Abstract
In this study we used Roche's 454 sequencing to develop genetic markers for Coenagrion scitulum, a Mediterranean damselfly currently expanding its range northward. With a modest amount of sequencing we detected 6,318 potential microsatellite markers and 832 putative single nucleotide polymorphisms (SNPs). From the potential microsatellite markers we developed thirteen novel microsatellite markers. Among other applications, these markers can be used to unravel the micro-evolutionary consequences of range expansion in this species.
| Item ID: | 27684 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1877-7260 |
| Keywords: | coenagrion; damselfly; range expansion; microsatellite; SNP; 454 sequencing |
| Date Deposited: | 18 Sep 2013 03:05 |
| FoR Codes: | 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 50% 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060408 Genomics @ 50% |
| SEO Codes: | 96 ENVIRONMENT > 9603 Climate and Climate Change > 960305 Ecosystem Adaptation to Climate Change @ 100% |
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