A rapid genetic assay for the identification of the most common Pocillopora damicornis genetic lineages on the Great Barrier Reef

Torda, Gergely, Schmidt-Roach, Sebastian, Peplow, Lesa M., Lundgren, Petra, and van Oppen, Madeleine J.H. (2013) A rapid genetic assay for the identification of the most common Pocillopora damicornis genetic lineages on the Great Barrier Reef. PLoS One, 8 (3). e58447. pp. 1-5.

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Abstract

Pocillopora damicornis (Linnaeus, 1758; Scleractinia, Pocilloporidae) has recently been found to comprise at least five distinct genetic lineages in Eastern Australia, some of which likely represent cryptic species. Due to similar and plastic gross morphology of these lineages, field identification is often difficult. Here we present a quick, cost effective genetic assay as well as three novel microsatellite markers that distinguish the two most common lineages found on the Great Barrier Reef. The assay is based on PCR amplification of two regions within the mitochondrial putative control region, which show consistent and easily identifiable fragment size differences for the two genetic lineages after Alu1 restriction enzyme digestion of the amplicons.

Item ID: 27458
Item Type: Article (Research - C1)
ISSN: 1932-6203
Additional Information:

© 2013 Torda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funders: Australian Government’s Marine and Tropical Sciences Research Fund, AIMS@JCU
Date Deposited: 05 Jun 2013 09:40
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 30%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 30%
06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060301 Animal Systematics and Taxonomy @ 40%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%
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