Analysis of cyclotides in Viola ignobilis by Nano liquid chromatography fourier transform mass spectrometry

Hashempour, Hossein, Ghassempour, Alireza, Daly, Norelle, Spengler, Bernhard, and Römpp, Andreas (2011) Analysis of cyclotides in Viola ignobilis by Nano liquid chromatography fourier transform mass spectrometry. Protein and Peptide Letters, 18 (7). pp. 747-752.

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Abstract

Cyclotides are macrocyclic knotted peptides originating from plants. They are extremely stable and have a range of bioactivities including anti-HIV and insecticidal activity. Given the stability of the cyclotide framework, there is interest in using these peptides as scaffolds in drug design. In the current study, we have shown that nano-LC Fourier transform mass spectrometry (FTMS) is an effective method of analyzing cyclotides in plants. In addition, we have used this technique to find cyclotides in a novel species, Viola ignobilis (Violaceae plant family), which was collected from the West Azerbaijan province of Iran. Varv peptide A, cycloviolacin B2, and cycloviolacin O8 were found in this species. This study provides a novel method for directly analyzing cyclotide sequences without enzymatic digestion and further information regarding the distribution of cyclotides in plant species.

Item ID: 27032
Item Type: Article (Research - C1)
ISSN: 1875-5305
Keywords: cyclic peptide, cyclotide, nano-LC FTMS, Varv peptide A, Viola ignobilis
Date Deposited: 03 Jun 2013 04:53
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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