Analysis of mutants disrupted in bacillithiol metabolism in Staphylococcus aureus

Rajkarnikar, Arishma, Strankman, Andrew, Duran, Shayla, Vargas, Derek, Roberts, Alexandra A., Barretto, Kathryn, Upton, Heather, Hamilton, Christopher J., and Rawat, Mamta (2013) Analysis of mutants disrupted in bacillithiol metabolism in Staphylococcus aureus. Biochemical and Biophysical Research Communications, 436 (2). pp. 128-133.

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Abstract

Bacillithiol (BSH), an α-anomeric glycoside of L-cysteinyl-D-glucosaminyl-L-malate, is a major low molecular weight thiol found in low GC Gram-positive bacteria, such as Staphylococcus aureus. Like other low molecular weight thiols, BSH is likely involved in protection against a number of stresses. We examined S. aureus transposon mutants disrupted in each of the three genes associated with BSH biosynthesis. These mutants are sensitive to alkylating stress, oxidative stress, and metal stress indicating that BSH and BSH-dependent enzymes are involved in protection of S. aureus. We further demonstrate that BshB, a deacetylase involved in the second step of BSH biosynthesis, also acts as a BSH conjugate amidase and identify S. aureus USA 300 LAC 2626 as a BSH-S-transferase, which is able to conjugate chlorodinitrobenzene, cerulenin, and rifamycin to BSH.

Item ID: 26895
Item Type: Article (Refereed Research - C1)
Keywords: bacillithiol; bacillthiol-S-transferase; bacillithiol conjugate amidase; thiols; Staphylococcus aureus; Bacillus subtilis
ISSN: 1090-2104
Funders: National Institutes of Health (NIH)
Projects and Grants: 1SC3GM100855
Date Deposited: 20 Aug 2013 00:08
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 40%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060501 Bacteriology @ 40%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060107 Enzymes @ 20%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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