Development and characterization of microsatellite markers for parentage analyses of the coral reef damselfish (Pomacentrus amboinensis: Pomacentridae)
Munkres, Katherine P., Bay, Line K., Jerry, Dean R., McCormick, Mark I., and van Herwerden, Lynne (2007) Development and characterization of microsatellite markers for parentage analyses of the coral reef damselfish (Pomacentrus amboinensis: Pomacentridae). Conservation Genetics, 8 (4). pp. 987-990.
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Abstract
Five new polymorphic microsatellite loci were developed for the coral reef damselfish Pomacentrus amboinensis. Twenty-four individuals from two Great Barrier Reef populations were genotyped at the five loci, with numbers of alleles per locus ranging from 6–23 and observed heterozygosity between 0.42–0.92. In addition, the cross-species testing of six primers developed for Stegastes partitus revealed one primer (SpGATA40) that was also polymorphic for P. amboinensis. Due to high levels of polymorphism (≥14 alleles) in at least four of the six loci and a high proportion of tetranucleotide repeats, these microsatellite markers should be useful for parentage assignment as well as other investigations of individual relatedness.
| Item ID: | 2646 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1572-9737 |
| Keywords: | damselfish; microsatellite analysis; Pomacentridae; coral reef; genetic diversity |
| Date Deposited: | 29 Jul 2009 02:53 |
| FoR Codes: | 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100% |
| SEO Codes: | 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100% |
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