Oocyst wall formation and composition in coccidian parasites

Mai, Kelly, Sharman, Philippa A., Walker, Robert A., Katrib, Marilyn, De Souza, David, McConville, Malcolm J., Wallach, Michael G., Belli , Sabina I., Ferguson, David J.P., and Smith, Nicholas C. (2009) Oocyst wall formation and composition in coccidian parasites. Memorias do Instituto Oswaldo Cruz , 104 (2). pp. 281-289.

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Abstract

The oocyst wall of coccidian parasites is a robust structure that is resistant to a variety of environmental and chemical insults. This resilience allows oocysts to survive for long periods, facilitating transmission from host to host. The wall is bilayered and is formed by the sequential release of the contents of two specialized organelles - wall forming body 1 and wall forming body 2 - found in the macrogametocyte stage of Coccidia. The oocyst wall is over 90% protein but few of these proteins have been studied. One group is cysteine-rich and may be presumed to crosslink via disulphide bridges, though this is yet to be investigated. Another group of wall proteins is rich in tyrosine. These proteins, which range in size from 8-31 kDa, are derived from larger precursors of 56 and 82 kDa found in the wall forming bodies. Proteases may catalyze processing of the precursors into tyrosine-rich peptides, which are then oxidatively crosslinked in a reaction catalyzed by peroxidases. In support of this hypothesis, the oocyst wall has high levels of dityrosine bonds. These dityrosine crosslinked proteins may provide a structural matrix for assembly of the oocyst wall and contribute to its resilience.

Item ID: 26390
Item Type: Article (Research - C1)
ISSN: 0074-0276
Keywords: coccidia, oocyst, dityrosine crosslinking, toxoplasma, Eimeria
Date Deposited: 05 Jun 2013 02:21
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 30%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 20%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 40%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 30%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 30%
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