Mutation scanning-based analysis of anisakid larvae from Sillago flindersi from Bass Strait, Australia

Jabbar, Abdul, Khoon, Avalene T.W., Hui, Teo X. , Schaeffner, Bjoern C., Jex, Aaron R., Nolan, Matthew J., Lopata, Andreas, Gasser, Robin B., and Beveridge, Ian (2012) Mutation scanning-based analysis of anisakid larvae from Sillago flindersi from Bass Strait, Australia. Electrophoresis, 33 (3). pp. 499-505.

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Abstract

Anisakidosis is an important fish-borne disease caused by the larvae of anisakid nematodes, which affects humans and a range of other animals. The accurate identification of members of this nematode group is central to investigating the epidemiology of the parasites and in the surveillance and control of anisakidosis. It is now well known that morphological identification alone does not allow specific identification, particularly of larval stages. To better understand the epidemiology of anisakid nematodes in southern Australian fishes and the potential risks posed to human health, a survey of 50 specimens of the commercially important fish, Sillago flindersi, from Bass Strait, Australia was conducted. We characterised anisakid larvae by PCR-coupled mutation scanning, sequencing and phylogenetic analyses of the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA. This study revealed that 92% of the S. flindersi examined were infected with anisakids (n=194), which were represented by seven genotypes. Phylogenetic analyses of the genotypes defined herein, together with reference sequence for Anisakis pegreffii and Hysterothylacium sp. from public databases (i.e. GenBank), revealed the presence of A. pegreffii (n=24), Hysterothylacium larval type IV (n=90) and Hysterothylacium larval type VIII (n=80) in S. flindersi. Thus, the PCR-coupled mutation scanning approach employed herein is an effective tool for the genetic characterisation of anisakid nematodes for diagnostic and analytical purposes (nucleotide sequences reported in this paper are available in the GenBank database under accession nos. JN631796-809).

Item ID: 22680
Item Type: Article (Research - C1)
ISSN: 1522-2683
Keywords: Anisakidae, internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA (rDNA), nematoda, single-strand conformation polymorphism
Funders: Australian Biological Resources Study, Australian Research Council (ARC)
Date Deposited: 01 Aug 2012 09:50
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 34%
11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110307 Gastroenterology and Hepatology @ 33%
03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030403 Characterisation of Biological Macromolecules @ 33%
SEO Codes: 92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920406 Food Safety @ 34%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 33%
92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920404 Disease Distribution and Transmission (incl. Surveillance and Response) @ 33%
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