Polymorphic repetitive loci of the amphibian pathogen Batrachochytrium dendrobatidis

Garland, Stephen, James, Timothy Y. , Blair, David, Berger, Lee, and Skerratt, Lee F. (2011) Polymorphic repetitive loci of the amphibian pathogen Batrachochytrium dendrobatidis. Diseases of Aquatic Organisms, 97 (1). pp. 1-9.

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Batrachochytrium dendrobatidis (Bd), the cause of a fatal fungal skin disease of amphibians that has led to massive die-offs, global declines and extinctions, has spread internationally as a pandemic clone with low genetic diversity. A need exists to develop highly polymorphic markers to determine centers of origin and patterns of spread to assist in the development of management strategies. Comparison of paralogous sequences, obtained from the 2 sequenced Bd genomes, indicates useful levels of inter-strain polymorphism in repetitive fragments. We assessed 6 repetitive loci for variation within and among Australian isolates using standard fragment analysis and capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) analysis. Confirmation of inter-isolate polymorphism was achieved for 2 marker systems, highlighting the potential of repetitive loci for the development of polymorphic markers in Bd. In addition, we found that repetitive loci in Bd include possible orthologs of virulence-related genes from pathogenic fungi.

Item ID: 20659
Item Type: Article (Research - C1)
ISSN: 1616-1580
Keywords: Batrachochytrium dendrobatidis, chytrid, molecular strain typing, SSCP
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Supplemental material available at http://dx.doi.org/10.3354/dao02396

Date Deposited: 06 Mar 2012 02:38
FoR Codes: 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060309 Phylogeny and Comparative Analysis @ 50%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 50%
SEO Codes: 96 ENVIRONMENT > 9604 Control of Pests, Diseases and Exotic Species > 960405 Control of Pests, Diseases and Exotic Species at Regional or Larger Scales @ 50%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960805 Flora, Fauna and Biodiversity at Regional or Larger Scales @ 50%
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