Identification of twenty one microsatellite loci for conservation genetic studies of the endemic butterflyfish Chaetodon tricinctus

van der Meer, Martin H., Gardiner, Michael G., Hobbs, Jean-Paul A., Pratchett, Morgan, and van Herwerden, Lynne (2012) Identification of twenty one microsatellite loci for conservation genetic studies of the endemic butterflyfish Chaetodon tricinctus. Conservation Genetics, 4 (2). pp. 243-246.

[img] PDF (Published Version) - Published Version
Restricted to Repository staff only

View at Publisher Website: http://dx.doi.org/10.1007/s12686-011-951...
 
1
2


Abstract

Coral reef habitats are increasingly under threat from global warming and this has influenced the associated reef fish communities. Chaetodon tricinctus is a butterflyfish endemic to the offshore reefs of Elizabeth Reef, Middleton Reef, Lord Howe Island and Norfolk Island off Australia's east coast. It is a highly specialised coral reef fish that is thought to rely on Acroporid coral species exclusively for food and shelter. We developed primers for twenty one microsatellite loci to reveal gene flow, population genetic structure and genetic diversity within and among these three reefs. Observed heterozygosities ranged from 0.185 to 0.964 and expected heterozygosities ranged from 0.230 to 0.889 in 30 individuals from Middleton Reef. When cross tested with Chaetodon trifascialis, a closely related species, there was poor amplification success and only a moderate level of polymorphism. Therefore, although these loci will be useful in C. tricinctus, it is unlikely that they can be used on other related butterflyfishes.

Item ID: 19588
Item Type: Article (Research - C1)
ISSN: 1572-9737
Keywords: coral reef fish, island endemics, genetic diversity, cross-species amplification
Date Deposited: 16 Jan 2012 04:52
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Downloads: Total: 2
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page