Detection and quantification of the coral pathogen vibrio coralliilyticus by real-time PCR with TaqMan fluorescent probes
Pollock, F. Joseph, Morris, Pamela J., Willis, Bette L., and Bourne, David G. (2010) Detection and quantification of the coral pathogen vibrio coralliilyticus by real-time PCR with TaqMan fluorescent probes. Applied and Environmental Microbiology, 76 (15). pp. 5282-5286.
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Abstract
A real-time quantitative PCR-based detection assay targeting the dnaJ gene (encoding heat shock protein 40) of the coral pathogen Vibrio coralliilyticus was developed. The assay is sensitive, detecting as little as 1 CFU per ml in seawater and 104 CFU per cm2 of coral tissue. Moreover, inhibition by DNA and cells derived from bacteria other than V. coralliilyticus was minimal. This assay represents a novel approach to coral disease diagnosis that will advance the field of coral disease research.
| Item ID: | 16332 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1098-5336 |
| Date Deposited: | 28 Apr 2011 05:28 |
| FoR Codes: | 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 100% |
| SEO Codes: | 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50% 96 ENVIRONMENT > 9604 Control of Pests, Diseases and Exotic Species > 960407 Control of Pests, Diseases and Exotic Species in Marine Environments @ 50% |
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