Proinflammatory cytokine mRNA responses in experimental Burkholderia pseudomallei infection in mice

Ulett, Glen C., Ketheesan, Natkunam, and Hirst, Robert G. (2000) Proinflammatory cytokine mRNA responses in experimental Burkholderia pseudomallei infection in mice. Acta Tropica, 74 (2-3). pp. 229-234.

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Melioidosis is a potentially fatal disease of both human and animals caused by the bacterium Burkholderia pseudomallei. Disease is endemic in tropical and subtropical regions of Southeast Asia and Northern Australia. The pathogenesis of melioidosis is poorly understood. In particular, the host responses that occur following infection, and the specific host-pathogen interactions that result in the development of either acute or chronic infection are unclear. Using an established murine model, we investigated early proinflammatory cytokine responses believed to be critical in the development of acute and chronic B. pseudomallei infection. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to assess levels of mRNA for tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6) in the liver of mice following infection. We demonstrate that the level of mRNA for these cytokines increase moderately in chronic infection in C57BL/6 mice. However, in acute infection in BALB/c mice, mRNA responses for these cytokines were shown to be comparatively greater. These results demonstrate that early proinflammatory cytokine responses are important in the immunopathogenesis of melioidosis.

Item ID: 12731
Item Type: Article (Research - C1)
ISSN: 1873-6254
Keywords: Burkholderia pseudomallei; cytokine; melioidosis
Date Deposited: 03 Jul 2012 05:23
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110802 Medical Infection Agents (incl Prions) @ 33%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 34%
86 MANUFACTURING > 8609 Veterinary Pharmaceutical Products > 860901 Veterinary Biological Preventatives (e.g. Vaccines) @ 33%
86 MANUFACTURING > 8609 Veterinary Pharmaceutical Products > 860903 Veterinary Pharmaceutical Treatments (e.g. Antibiotics) @ 33%
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