Synthesis of bifunctional saxitoxin analogues by biotinylation

Robillot, Cedric, Kineavy, Daniel, Burnell, James, and Llewellyn, Lyndon E. (2009) Synthesis of bifunctional saxitoxin analogues by biotinylation. Toxicon, 53 (4). pp. 460-465.

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Abstract

Saxitoxin (STX) contaminates seafood and freshwater catchments worldwide. Conjugation of STX with biotin would enable new biochemical methods to quantitate STX and its analogues as well as diversify its utility as a research tool. We conjugated biotin at the region of the toxin normally occupied by a carbamoyl and this conjugate could concurrently bind both avidin/streptavidin and saxiphilin. Increasing the length of the linker between biotin and the STX portion of the semisynthetic analogue increased potency of saxiphilin binding of the STX moiety.

Item ID: 8420
Item Type: Article (Refereed Research - C1)
Keywords: saxitoxin; saxiphilin; biotin; avidin; streptavidin; conjugation
ISSN: 1879-3150
Date Deposited: 18 Feb 2010 04:27
FoR Codes: 03 CHEMICAL SCIENCES > 0305 Organic Chemistry > 030502 Natural Products Chemistry @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
Citation Count from Web of Science Web of Science 5
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