Pathogenesis of crown-of-thorns starfish (Acanthaster planci L)

Rivera Posada, Jairo A. (2012) Pathogenesis of crown-of-thorns starfish (Acanthaster planci L). PhD thesis, James Cook University.

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Abstract

Outbreaks of the crown-of-thorns starfish, Acanthaster planci (L.), represent one of the most significant biological disturbances on coral reefs, contributing greatly to widespread habitat degradation across the Indo-Pacific. While the cause(s) of outbreaks are still being debated, an equally important question is what causes population declines at the end of outbreaks. Like all echinoderms, A. planci appear to be susceptible to disease, which may explain sudden declines in abundance that have been observed in the wild, as well as providing a unique opportunity to potentially control starfish populations. The purpose of this thesis was to document potentially pathogenic organisms that are normally associated with A. planci, as well as describing the pathological progression of artifially-induced disease, following injection of Thiosulfate-citratebile-sucrose (TCBS).

Thiosulfate-citrate-bile-sucrose (TCBS) agar is a selective media culture that inhibits gram-positive organisms, suppresses coliforms, and allows selective growth of Vibrio bacteria. These bacteria constitute an important part of the bacterial microflora of numerous marine animals, and are also recognized as important pathogens of echinoderms and other estuarine animals. This study showed that injection of TCBS broth into A. planci organs induced disease characterized by skin lesions, loss of body turgor, matting of spines, and accumulation of mucus on spine tips. All starfish then died within 24 hours. TCBS broth promoted population growth of naturally occurring Vibrio spp., leading to an imbalance in natural symbiont communities. Moreover, diseased starfish often infected seemingly healthy A. planci that were either in direct contact or in very close proximity.

To identify potential causative agents of observed disease, and specifically identify all naturally occurring bacteria associated with A. planci, starfish were collected from two distinct locations in the western Pacific; Lizard Island (Great Barrier Reef, Australia) and Guam (USA, Western Pacific Ocean). A polyphasic approach, involving histology, scanning electron microscopy (SEM), biochemical profiling using the bacterial API identification system, PCR amplification and sequencing of 16S rRNA, topA (topoisomerase I) and mreB (rod shaping protein MreB) genes, was used to identify all bacterial isolated. The most significant bacteria isolated from A. planci were V. rotiferianus, V. harveyi, V. owensii, Photobacterium eurosenbergii, V. fortis, V. natriegens with sequences identities of 99%-100% for 16S rRNA, topA, and mreB. Specific bacteria isolated from infected tissues were V. rotiferianus, V. owensii and V. harveyi, which are considered as the most likely causative agents of observed disease.

Histological changes in tissues of A. planci following TCBS injection were assessed using conventional and scanning electron microscopy (SEM). Digestive glands were processed and stained with hematoxylin and eosin (H&E) to describe the histological architecture of the intestinal epithelium. Subsequent comparison of healthy versus infected tissues and Gram stains were carried out to confirm bacterial occurrence on infected tissues, characterize the structural changes induced by bacterial communities in COTS tissues and to determine if the histopathological changes of intestinal tissues were consistent with Vibrio infection. TCBS injections induced marked epithelial desquamation, hypertrophy and hypersecretion of glandular cells, epithelial cell destruction, pyknosis, reduction of thickness and disorganization of connective tissue and associated nerve plexus, presence of bacterial colonies, irregular eosinophilic foci in glandular cells, brush border disruption, atrophy and detachment of intestinal microvilli and cell debris in the lumen. All these changes were attributed to a fulminating systemic dysbiosis and were consistent with Vibrio infections.

Standard histological procedures used to test for the presence of bacteria are often ineffective for marine organisms. As such, this study developed modified techniques to assess the presence of Vibrio bacteria and the preservation of A. planci delicate tissues. Detection of Vibrio bacteria was improved by the (1) use of short washes before fixation (2) the implementation of short cycles in the processing step; (3) embedding samples in agar prior to automated processing. The use of short cycles also decreased the amount of epithelial desquamation of COTS digestive glands. The study contributes to the standardization of histological techniques and biochemical test (API strips) for partial identification of marine bacteria, ensuring more accurate results, improving performance, enhancing reproducibility and increasing efficiency compared to standard operating procedures.

In order to reverse sustained and ongoing degradation of reef habitat, increasing attention is being given to management and control of A. planci outbreaks. Previous control methods, such as hand collecting individual starfish, are extremely labour intensive and often ineffective in either eradicating the coral-feeding starfish or preventing extensive coral loss. As a first step towards assessing whether injections of thiosulfate-citrate-bile-sucrose agar (TCBS) culture medium could be used to eradicate A. planci, especially during population outbreaks, we exposed a range of echinoderms to diseased starfish within a closed environment, and also compared naturally occurring bacteria across these echiinoderms. Vibrio rotiferianus, which was reported as a likely pathogen isolated from experimentally infected A. planci, was recovered from Linckia guildingi. Moreover, several L. guildingi exhibited skin lesions after several days of direct contact with sick Acanthasther planci. However, unlike infected A. planci, which all died within 48 hrs, all L. guildingi starfish fully recovered after 53 days. Further studies need to be carried out to test for cross-infection of Vibrio bacteria isolated from sick A. planci to corals, fishes and other echinoderms.

To better understand the specific effects of thiosulfate-citrate-bile-sucrose agar (TCBS) on A. planci, we tested responses of A. planci to individual components of TCBS culture medium. Four out of nine TCBS chemical ingredients tested induced allergic reactions and death in A. planci. Peptone 10 g l⁻ ¹ and oxgall 8 g l⁻ ¹ induced 100% mortality, while yeast extract and agar induced death in 40% and 20% of starfish, respectively, 48h after injection. Peptone was evaluated at three different concentrations (10g, 5g, and 1g l⁻ ¹). Peptone 10 g l⁻ ¹ induced 100% mortality, peptone 5 g l⁻ ¹ killed 60% of injected starfish, and peptone 1 g l⁻ ¹ induced death in only 20% of starfish, indicating that toxicity of peptone is concentration dependent. Sodium citrate induced moderate mucus production, but disease did not progress and all starfish completely recovered after 52 h. The remaining chemicals tested, sodium thiosulfate, ferric citrate, mix of sodium thiosulfate + ferric citrate, sucrose and sodium chloride did not produce any kind of clinical signs of disease. This study reported four new components that induced disease and death in A. planci. Peptone, oxgall, and yeast are potentially useful in controlling outbreaks because these simple protein extracts can be safer to use compared to previously used noxious chemicals. In addition, lowered concentrations are required to kill A. planci, potentially increasing efficiency and effectiveness of established control programs.

Item ID: 23660
Item Type: Thesis (PhD)
Keywords: Acanthaster planci; coral reefs; corallivores; COTS; Crown-of-thorns starfish; disease; disturbance; histology techniques; histopathology; Linckia guildingi; management and control; marine pests; marine bacteria; outbreaks; pathogenic bacteria; pathogenic microorganisms; pathogens; pathological histology; TCBS agar; thiosulfate-citrate-bile salts-sucrose agar; vibrio bacteria; vibrio infections
Additional Information:

Publications arising from this thesis are available from the Related URLs field. The publications are:

Rivera Posada, J.A., Pratchett, M., Cano Gomez, A., Arango Gomez, J.D., and Owens, L. (2011) Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). I. Disease induction. Diseases of Aquatic Organisms, 97 (2). pp. 85-94.

Rivera Posada, J.A., Pratchett, M., Cano Gomez, A., Arango-Gomez, J.D., and Owens, L. (2011) Refined identification of Vibrio bacterial flora from Acanthasther planci based on biochemical profiling and analysis of housekeeping genes. Diseases of Aquatic Organisms, 96 (2). pp. 113-123.

Rivera Posada, J.A., Pratchett, M., and Owens, L. (2011) Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). II. Histopathological changes. Diseases of Aquatic Organisms, 97 (2). pp. 95-102.

Caballes C.F., Schupp P.J., Pratchett M.S., Rivera-Posada J.A. (2012) Interspecific transmission and recovery of TCBS-induced disease between Acanthaster planci and Linckia guildingi. Diseases of Aquatic Organisms, 100 (3). pp. 263-267.

Rivera-Posada J.A., Owens L., Caballes C.F., Pratchett M. (2012). The Role of protein extracts in the induction of disease in Acanthaster planci. Journal of Experimental Marine Biology and Ecology, DOI: 10.1016/j.jembe.2012.06.008.

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Funders: ARC Centre of Excellence for Coral Reef Studies
Date Deposited: 17 Oct 2012 04:04
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 50%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060501 Bacteriology @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960503 Ecosystem Assessment and Management of Coastal and Estuarine Environments @ 50%
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