Possible link between glycolysis and apoptosis induced by sodium fluoride

Otsuki, S., Morshed, S.R.M., Chowdhury, S.A., Takayama, F., Satoh, T., Hashimoto, K., Sugiyama, K., Amano, O., Yasui, T., Yokote, Y., Akahane, K., and Sakagami, H. (2005) Possible link between glycolysis and apoptosis induced by sodium fluoride. Journal of Dental Research, 84 (10). pp. 919-923.

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Fluoride has been used to prevent caries in the dentition, but the possible underlying mechanisms of cytotoxicity induction by this compound are still unclear. Since fluoride is known as an inhibitor of glycolytic enzymes, we investigated the possible connection between NaF-induced apoptosis and glycolysis in human promyelocytic leukemia HL-60 cells. NaF-induced apoptotic cell death is characterized by caspase activation, internucleosomal DNA fragmentation, loss of mitochondrial membrane potential, and production of apoptotic bodies. Higher activation of caspases-3 and -9, as compared with that of caspase-8, suggested the involvement of an extrinsic pathway. Utilization of glucose was nearly halted by NaF, whereas that of glutamine was rather enhanced. NaF enhanced the expression of Bad protein, but not that of Bcl-2 and Bax proteins, and reduced HIF-1 mRNA expression. Analysis of these data suggests a possible link between glycolysis and apoptosis.

Item ID: 988
Item Type: Article (Research - C1)
ISSN: 0022-0345
Keywords: apoptosis, glycolysis, bad protein, fluoride
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Copyright © 2005, IADR Journals Online by International & American Associations for Dental Research.

Date Deposited: 01 Nov 2006
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1105 Dentistry @ 0%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060104 Cell Metabolism @ 0%
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