Characterization of monoclonal antibodies that recognize the Eimeria tenella Microneme Protein MIC2

Sasai, Kazumi, Fetterer, Raymond H., Lillehoj, Hyun, Matsuura, Satomi, Constantinoiu, Constantin C., Matsubayashi, Makoto, Tani, Hiroyuki, and Baba, Eiichiroh (2008) Characterization of monoclonal antibodies that recognize the Eimeria tenella Microneme Protein MIC2. Journal of Parasitology, 94 (6). pp. 1432-1434.

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Abstract

The apicomplexan pathogens of Eimeria tenella cause coccidiosis, an intestinal disease of chickens that has a major economic impact on the poultry industry. Members of Apicomplexa share an assortment of unique secretory organelles (rhoptries, micronemes, and dense granules) that mediate invasion of host cells and the formation and modification of the parasitophorous vacuole. Among these, microneme protein 2 from E. tenella (EtMIC2) has a putative function in parasite adhesion to the host cell to initiate the invasion process. To investigate the role of EtMIC2 in host parasite interactions, the production and characterization of 12 monoclonal antibodies (mabs) produced against recombinant EtMIC2 proteins is described. In an immunofluorescence assay, all mabs reacted with molecules belonging to the apical complex of sporozoites and merozoites of E. tenella, E. acervulina, and E. maxima. By Western blot analysis, the mabs identified a developmentally regulated protein of 42 kDa corresponding to EtMIC 2 and cross-reacted with proteins in developmental stages of E. acervulina. Collectively, these mabs are useful tools for the detailed investigation of the characterization of EtMIC2-related proteins in Eimeria species.

Item ID: 9793
Item Type: Article (Research - C1)
ISSN: 1937-2345
Date Deposited: 30 Mar 2010 21:57
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070705 Veterinary Immunology @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 100%
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