Billah, Mutasim, Karmakar, Sumon, Mina, Fahmida Begum, Haque, Md. Nazmul, Rashid, Md. M., Hasan, Md. Faruk, Acharjee, Uzzal Kumar, and Talukder, Mamunur Rashid (2021) Investigation of mechanisms involved in seed germination enhancement, enzymatic activity and seedling growth of rice (Oryza Sativa L.) using LPDBD (Ar+Air) plasma. Archives of Biochemistry and Biophysics, 698. 108726.

PDF (Published Version) - Published Version Restricted to Repository staff only

Abstract

The study investigates the effect of LPDBD (Low Pressure Dielectric Barrier Discharge) (Ar + Air) plasma on seed germination, seedling growth and antioxidant enzyme activity of rice. Rice seeds were treated with LPDBD (Ar + Air) plasma for 2min, 4min, 6min, 8min and 10min. Seed germination rate, seedling growth, total chlorophyll content, enzymatic activity, total soluble sugar and protein concentration were increased in plants grown from the LPDBD (Ar + Air) plasma treated seeds. It was observed that the sprouting of seeds and the growth of seedlings of rice depends on the feed gases used to generate plasma and plasma processing time. In the case of plantlets germinated from the plasma-treated seeds of rice, the H2O2 level was increased significantly both in leaves and roots for 6min, 8min and 10min treatment respectively. No significant change was observed in Nitric Oxide (NO) concentration in seed, leaf, or root of plants grown from LPDBD (Ar + Air) plasma-treated seeds. The amount of total soluble sugar and protein increased significantly in the case of 2min, 4min, 6min, 8min and 10min seed treatment. Although plants exhibited no significant increase in APX activities, but a significant increase of CAT and SOD activity in the leaf and root was found. This study reveals that LPDBD (Ar + Air) plasma is involved in the elevation of ROS species in leaf and root of rice plants which is tightly regulated by the upregulation of CAT activity that ultimately enhances the seed germination and growth of rice plantlets.

Item ID:	93889
Item Type:	Article (Research - C1)
ISSN:	1096-0384
Copyright Information:	© 2020 Elsevier Inc. All rights reserved.
Date Deposited:	02 Sep 2026 03:36
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3001 Agricultural biotechnology > 300103 Agricultural molecular engineering of nucleic acids and proteins @ 50% 31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310803 Plant cell and molecular biology @ 50%
SEO Codes:	26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2601 Environmentally sustainable plant production > 260199 Environmentally sustainable plant production not elsewhere classified @ 50% 26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2603 Grains and seeds > 260399 Grains and seeds not elsewhere classified @ 50%
	More Statistics