Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.)

Hasan, Md, Ferdous, Jannatul, Dutta, Amit, Mina, Fahmida Begum, Karmakar, Sumon, and Sikdar, Biswanath (2023) Detection and biological control measures of anthracnose causing fungus isolated from Citrus limon (L.). Journal of Advanced Biotechnology and Experimental Therapeutics, 6 (1). pp. 58-66.

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Abstract

Anthracnose is one of the most devastating fungi causing twig dieback and postharvest fruit decay. Present study was aimed to identification of anthracnose fungus. Infected leaves of Citrus limon were collected and cultured on potato dextrose agar (PDA) media for pathogenic fungus isolation. The isolated fungal pure culture was characterized by physiological and morphological characterization methods. Biological control measures of the fungus were evaluated by disc diffusion methods. The highest growth and development of isolated fungus was detected in PDA media at pH 7 in fructose as the best carbon source and 0.05gm NaCl concentrations at 37°C. Pathogenicity potency of isolate was performed on lemon, orange and malta, and showed typical anthracnose symptoms after incubation at 25°C for 5 days. For antifungal activity, 200µgm/disc methanolic extract of Psidium guajava showed 14.33±0.66 mm inhibition zone against the isolated fungus. From the present investigations, identified anthracnose causing fungus and it’s controlling techniques may help for further research for the isolation of drugs related compound for controlling this disease.

Item ID: 93886
Item Type: Article (Research - C1)
ISSN: 2616-4760
Copyright Information: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Date Deposited: 02 Sep 2026 04:14
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300401 Agrochemicals and biocides (incl. application) @ 40%
31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310705 Mycology @ 60%
SEO Codes: 26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2601 Environmentally sustainable plant production > 260199 Environmentally sustainable plant production not elsewhere classified @ 100%
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