Tracking dendritic cells in vivo

Rush, Catherine M., and Brewer, James M. (2010) Tracking dendritic cells in vivo. In: Davies, Gwyn, (ed.) Vaccine Adjuvants: methods and protocols. Methods in Molecular Miology (626). Humana Press, New York, USA, pp. 169-185.

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The reasons why certain vaccine adjuvants and/or delivery systems are more or less effective at inducing immune responses or promoting the preferential induction of particular types of response are unknown. While vaccine antigen discovery has benefited from a systematic approach, our limited understanding of the interactions of adjuvants with cells of the immune system has hampered rational adjuvant discovery and handicapped the development of new and more effective vaccines. It is well accepted that the component parts of the immune system do not work in isolation and their interactions occur in distinct and specialised micro- and macro-anatomical locations. Consequently, significant obstacles to the systematic investigation of adjuvant effects have been the complexity of the physiological environments that adjuvants interact with and the difticulty in directly investigating these interactions dynamically in vivo. Here we describe some of the immunological and microscopical techniques that have enabled the analysis of the immune cells and their interactions, in vivo, in real time. It is only by performing such detailed and fundamental studies in vivo that we can fully understand the cellular and molecular interactions that control the immune response. These types of systematic analyses of the events involved in adjuvant action are a prerequisite if we are truly to design, build and target vaccines effectively.

Item ID: 9309
Item Type: Book Chapter (Research - B1)
ISBN: 978-1-60761-584-2
Keywords: Immunology, adjuvants, dendritic cells, imaging, in vivo, vaccines, lymph nodes
Date Deposited: 09 Sep 2010 23:37
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 100%
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