Ross, Ailie, Wight, Joel C., Devi, Aruna, and Rudd, Donna (2026) Enhancing storage, transport and affordability of multicolour antibody cocktails for diagnostic flow cytometry: A novel approach. Australian Journal of Medical Sciences, 47 (2). pp. 48-58.

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Abstract

Flow cytometry is used extensively in pathology laboratories for the diagnosis of haematological malignancies and immune disorders. A major barrier to implementation in regional and remote resource-constrained areas is the cost and stability of multicolour antibody cocktails. To improve multicolour cocktail storage stability, we trialled a novel vitrification technique. Reagents were tested across a range of temperatures and storage durations designed to reflect extreme storage conditions in remote tropical climates. The vitrified product compared favourably with existing techniques under refrigerated and room-temperature conditions. Notably, superior stability was observed at moderate heat (40°C) representing ambient tropical conditions. In the extreme heat category (60°C), liquid cocktails failed within 2 hours. Vitrification ensured performance of antibody cocktails after 72 hours of exposure to extreme heat, at a fraction of the cost of similar preparations. This feasibility study indicates that the novel vitrification method described may protect antibody cocktails during storage and transport and warrants further development.

Item ID:	93062
Item Type:	Article (Research - C1)
ISSN:	1038-1643
Date Deposited:	30 Jul 2026 00:06
FoR Codes:	32 BIOMEDICAL AND CLINICAL SCIENCES > 3211 Oncology and carcinogenesis > 321102 Cancer diagnosis @ 50% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320402 Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies) @ 50%
SEO Codes:	24 MANUFACTURING > 2408 Human pharmaceutical products > 240802 Human diagnostics @ 100%
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