Assessment of a DNA vaccine encoding Burkholderia pseudomallei bacterioferritin

McAllister, Jane, Gauci, Penny, Lazzaroni, Sharon, Barnes, Jodie, Ketheesan, Natkunam, and Proll, David (2007) Assessment of a DNA vaccine encoding Burkholderia pseudomallei bacterioferritin. Report. Defence Science and Technology Organisation, Fishermans Bend, VIC, Australia.

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Abstract

Burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic in Southeast Asia and Northern Australia. The bacteria cause infection via subcutaneous or inhaled routes, resulting in either acute lethal sepsis or chronic and eventually fatal disease. Currently no licensed vaccine is available to provide protection against this pathogen. Intracellular enzymatic proteins of other bacterial species, such as the iron storage protein bacterioferritin, have been shown to be potent inducers of the immune response. In this study, a DNA vaccine encoding the B. pseudomallei bacterioferritin protein was constructed. The DNA vaccine was then used to immunise mice and analyse subsequent immune responses and protective capability following live challenge with B. pseudomallei. There was a substantial increase in anti-bacterioferritin IgG titers following immunisation, however the cellular response and survival following challenge was limited, suggesting that the vaccine may need to be used in conjunction with adjuvant such as CpG or in a multicomponent vaccine in order to increase protective capabilities.

Item ID: 9256
Item Type: Report (Report)
Date Deposited: 10 Sep 2010 05:37
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 60%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 40%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 50%
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