The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato
Garland, S., Sharman, M., Persley, D., and McGrath, D. (2005) The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato. Australian Journal of Agricultural Research, 56 (3). pp. 285-289.
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Abstract
Sw-5 is an important disease resistance gene of tomato, providing broad resistance to Tomato spotted wilt virus TSWV). A cleaved amplified polymorphic sequence (CAPS) marker, losely linked to the gene, has been reported. Although the Sw-5 locus has been characterised, a gene-specific marker has not been developed. This paper presents a PCR-based marker-system that consists of the co-amplification of a dominant marker representing the Sw-5 gene sequence, and the modified CAPS marker as a positive control and indicator of genotype.
| Item ID: | 9059 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1836-5795 |
| Keywords: | rapid DNA extraction |
| Date Deposited: | 15 Mar 2010 03:11 |
| FoR Codes: | 07 AGRICULTURAL AND VETERINARY SCIENCES > 0706 Horticultural Production > 070602 Horticultural Crop Improvement (Selection and Breeding) @ 100% |
| SEO Codes: | 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8202 Horticultural Crops > 820215 Vegetables @ 100% |
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