Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae)

McPherson, Hannah, Porter, Carolyn, Rymer, Paul D., Crayn, Darren M., and Rossetto, Maurizio (2009) Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae). Molecular Ecology Resources, 8 (4). pp. 867-869.

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Abstract

We identified 11 informative microsatellite loci in Tetratheca ericifolia from an (AG)n-enriched library. Using these loci on 32 individuals from two populations (16 individuals from each), we detected an average of 11.3 alleles per locus (range of five to 21, average expected heterozygosity of 0.728), of which 56% were unique to one population or the other. All loci were amplifiable in seven to 12 of a further 12 species of Tetratheca under the same reaction conditions. The markers will be useful tools for evolutionary studies of this Australian endemic group.

Item ID: 9046
Item Type: Article (Research - C1)
ISSN: 1755-0998
Keywords: conservation; cross-transferability; elaeocarpaceae; Tetratheca; tremandraceae
Date Deposited: 05 May 2010 05:31
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960806 Forest and Woodlands Flora, Fauna and Biodiversity @ 40%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960811 Sparseland, Permanent Grassland and Arid Zone Flora, Fauna and Biodiversity @ 40%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960810 Mountain and High Country Flora, Fauna and Biodiversity @ 20%
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