Khatun, Mst Muslima, Bhuia, Md Shimul, Rahaman, Mizanur, Kamli, Hossam, Ansari, Siddique Akber, and Islam, Muhammad Torequl (2025) Modulatory Sedative Activity of Helicide on Diazepam in Thiopental Sodium Mediated Sleeping Mice: An In Vivo Approach With Molecular Docking. Revista Brasileira De Farmacognosia, 35 (2). pp. 412-421.

PDF (Published Version) - Published Version Restricted to Repository staff only

Abstract

Insomnia is a common sleep disorder characterized by difficulties in falling asleep, staying asleep, or getting restful sleep. This study aimed to assess the sedative properties of helicide using thiopental sodium-induced sleep mice model. Helicide (5 and 10 mg/kg) and diazepam (2 mg/kg) were administered to the animals. After 30 min, thiopental sodium (20 mg/kg) was given (i.p.) to induce sleep, and onset and duration of their sleep were recorded over a 4-h period. Molecular docking was also performed to investigate the potential interactions of helicide with GABA<inf>A</inf> (PDB: 6X3X, containing α1 and β2 subunits) receptors and assess its pharmacokinetics and toxicity. The results demonstrated that helicide significantly (p > 0.05) extended the duration of sleep (174.8 ± 10.47 min) and provided fast sleeping onset (9.40 ± 1.71 min) compared to control. The combination of helicide and diazepam elevated sedation duration (191.40 ± 3.86 min) and lowered onset (5.20 ± 0.58 min), indicating a synergistic effect. Helicide exerted binding affinity of − 7.7 kcal/mol, while diazepam had a slightly higher affinity of − 8.7 kcal/mol with 6X3X. Helicide also displayed favorable pharmacokinetics and drug-likeness. Overall, Helicide exhibited a moderate sedative effect in the mice model, likely through its interaction with the GABAergic system.

Actions (Repository Staff Only)

Item Control Page