Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
Sadaow, Lakkhana, Rodpai, Rutchanee, Smout, Michael J., Nakajima, Rie, Boonroumkaew, Patcharaporn, Sotillo, Javier, Tedla, Bemnet A., Luvira, Vor, Kitkhuandee, Amnat, Paonariang, Krisada, Sukeepaisarnjaroen, Wattana, Yamasaki, Hiroshi, Suttiprapa, Sutas, Laha, Thewarach, Sripa, Banchob, de Assis, Rafael, Jain, Aarti, Ittiprasert, Wannaporn, Mann, Victoria H., Wong, Yide, Felgner, Philip L., Maleewong, Wanchai, Brindley, Paul J., Loukas, Alex, and Intapan, Pewpan M. (2025) Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma. Nature Communications, 16 (1). 5965.
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Abstract
Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer.
| Item ID: | 87749 |
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| Item Type: | Article (Research - C1) |
| ISSN: | 2041-1723 |
| Copyright Information: | ©The Author(s) 2025. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
| Date Deposited: | 12 Feb 2026 05:29 |
| FoR Codes: | 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320704 Medical parasitology @ 50% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3202 Clinical sciences > 320214 Nephrology and urology @ 50% |
| SEO Codes: | 20 HEALTH > 2001 Clinical health > 200101 Diagnosis of human diseases and conditions @ 100% |
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