A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion.

Kockisch, Sandra, Rees, Gareth D, Young, Simon A, Tsibouklis, John, and Smart, John D (2001) A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion. Journal of controlled release, 77 (1-2). pp. 1-6.

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Abstract

A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.

Item ID: 872
Item Type: Article (UNSPECIFIED)
ISSN: 0168-3659
Keywords: Bioadhesion, Oral cavity, Poly(acrylic acid), Chitosan
Additional Information:

© 2001 Elsevier : This journal is available online - use hypertext links above.

Date Deposited: 26 Oct 2006
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences @ 0%
Downloads: Total: 2
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