Talukder, S., Kerrisk, K.L., Gabai, G., Fukutomi, A., Celi, P., and UNSPECIFIED (2014) Milk oxidant and antioxidant status in dairy cows with ovulatory versus anovulatory oestrous cycles. In: [Presented at the 1st Dairycare Conference]. From: 1st Dairycare Conference, 22-23 August 2014, Copenhagen, Denmark.

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Abstract

The aim of this study was to evaluate changes in milk profiles of oxidative stress (OS) biomarkers in dairy cows with ovulatory and anovulatory oestrous cycles. Thirty (11 primiparous and 19 multiparous) healthy, lactating, cycling Holstein Friesian dairy cows averaging 60 ± 17 days in milk, and producing 33 ± 6 kg of milk per day were enrolled in this study. Composite milk samples were collected thrice weekly and assayed for progesterone(P4) concentration and the following OS biomarkers: superoxide dismutase (SOD), advanced oxidation protein products (AOPP), ceruloplasmin, glutathione (GSH), β-carotene and glutathione peroxidise (GSH-Px). Individual milk P4 profiles were constructed for each cow to characterise ovarian activity so that cows’ oestrus cycles could be identified as ovulatory (n = 20) or anovulatory (n =10). Oestrous cycle was categorised to different phases; oestrus (day 0), metoestrus (day 2-4), dioestrus (day 5-17) and prooestrus (day 17-20) to examine the changes of oxidants and antioxidant status across the different phases of the oestrous cycle. Day of ovulation was considered as day of oestrus (day 0).Differences in OS biomarkers were compared between two groups (ovulatory vs anovulatory) using linear mixed models with cow identification number being a random factor. Cows with ovulatory oestrous cycles presented significantly higher SOD levels compared to cows that did not ovulate (P< 0.05). On the other hand, GSH-Px and GSH concentrations were lower in ovulated cows compared to the an-ovulated cows (P< 0.05). The highest level of AOPP was noted during the prooestrus phase while β-carotene presented its lowest values during oestrous. It could be postulated that the elevated level of milk SOD and the observed lower level of GSH-Px and GSH in ovulating cows may be the essential events preceding the ovulatory response.

Item ID:	86341
Item Type:	Conference Item (Abstract / Summary)
Date Deposited:	24 Jul 2025 01:03
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300902 Veterinary anatomy and physiology @ 100%
SEO Codes:	10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1004 Livestock raising > 100402 Dairy cattle @ 100%
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