Turner, Benjamin L., Newman, Susan, Cheesman, Alex, and Reddy, K. Ramesh (2007) Sample Pretreatment and Phosphorus Speciation in Wetland Soils. Soil Science Society of America Journal, 71 (5). pp. 1538-1546.

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Abstract

We assessed the influence of sample pretreatment on the amounts and forms of P extracted in NaOH–EDTA (ethylenediamine tetraacetic acid) from a series of contrasting wetland soils from the Florida Everglades. Samples of unconsolidated benthic floc and underlying soil (0–10 cm) were extracted either fresh (overnight refrigeration only), air dried (10 d at ∼30°C), or frozen at −80°C and lyophilized (∼48 h), before extraction and solution 31P nuclear magnetic resonance (NMR) spectroscopy. Significant differences in total P extraction following pretreatment were detected for one out of four benthic floc samples and three out of four soil samples, although the changes were inconsistent: in two cases the total P extraction increased, while in two others it decreased. Assessment of the P composition by solution 31P NMR spectroscopy revealed differences among treatments, although these were mostly within the range of error associated with replicate analyses; however, DNA was not detected in a fresh sample of calcareous benthic floc, despite representing an important component of the organic P extracted from dried samples. The apparent sample-specific nature of the changes confirms the importance of carefully assessing pretreatment effects in studies of soil organic P in wetlands.

Item ID:	84407
Item Type:	Article (Research - C1)
ISSN:	1435-0661
Copyright Information:	All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.
Date Deposited:	13 Jan 2025 06:05
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