Mazumder, Sabuj Kanti, Ghaffar, Mazlan Abd., Das, Simon Kumar, and Alam, Md. Samsul (2014) Low occurrence of WSSV in Penaeus monodon nauplii and post-larvae produced from PCR-negative broodstocks. Aquaculture International, 23. pp. 1109-1123.

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Abstract

White spot syndrome virus (WSSV) is a devastating, infectious virus affecting farming tiger shrimp (Penaeus monodon). In efforts to limit trans-boundary movement of this pathogen and reduce the economic and socioeconomic impact of disease in shrimp culture, there is considerable scope for more effective use of DNA-based methods of pathogen detection. To assess the prevalence of the WSSV, broods, nauplii and post-larvae (PL) samples were collected from nine shrimp hatcheries located in the southeast and southwest regions of Bangladesh during the period from February 2008 to May 2014 and subjected to screening for WSSV by polymerase chain reaction (PCR) technique. The prevalence of WSSV during the 7-year period ranged from 48.15 to 62.99 %, 12.88 to 31.55 % and 4.03 to 8.89 % in broodstocks, nauplii and post-larvae, respectively. Out of a total of 5489 brood samples analyzed during the 7 years, 2,977 were found to be PCR positive for WSSV giving an overall prevalence of 54.24 %. The overall rates of PCR positive were 19.52 % for nauplii and 6.25 % for PL. The rates of infection in the nauplii and PL were much less than that observed in the broodstocks as the nauplii and PL samples were collected from broods found to be negative for WSSV by PCR. A follow-up study showed successful culture in about 87 % of the shrimp farms stocked with the PL that were PCR negative for WSSV. This study confirmed that PCR is an effective diagnostic tool for the screening of broodstocks before being used for spawning in the hatchery, to prevent vertical transmission of WSSV from parents to larvae. However, to avail the advantage of PCR screening for a risk-free crop, sources of potential horizontal contaminations should also be controlled.

Item ID:	84049
Item Type:	Article (Research - C1)
ISSN:	1573-143X
Keywords:	Broodstock, Hatcheries, Penaeus monodon, White spot syndrome virus, PCR
Related URLs:	https://link.springer.com/article/10.100...
Copyright Information:	© Springer International Publishing Switzerland 2014
Date Deposited:	19 Nov 2024 00:46
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