Kanti Nath, Babu, Gupta, Suman Das, Talukder, Saranika, Tonu, Nasrin Sultana, Raidal, Shane R., Forwood, Jade K., and Sarker, Subir (2024) Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia. Veterinary Medicine and Science, 10 (6).

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Abstract

Background: Birds are known to harbour many pathogens, including circovirus, herpesviruses, adenoviruses and Chlamydia psittaci. Some of these pose zoonotic risks, while others, such as beak and feather disease virus (BFDV), have a significant impact on the conservation of endangered bird species.

Objectives: This study was aimed to determine the faecal virome of a group of apparently healthy Monk parakeet using high-throughput sequencing.

Methods: Fresh faecal samples were collected from four Monk parakeets at a pet shop in Melbourne, Australia. Virus enrichment and nucleic acid extraction were performed on the faecal samples, followed by high-throughput sequencing at the Australian Genome Research Facility (AGRF).

Results: Utilising an established pipeline for high-throughput sequencing data analysis, this study revealed the presence of three viruses of the families Circoviridae, Parvoviridae and Adenoviridae. Subsequent sequence comparison and phylogenetic analyses further confirmed that the detected viruses belong to the genera Chaphamaparvovirus (unassigned species), Circovirus (species Circovirus parrot) and Siadenovirus (species Siadenovirus viridis).

Conclusion: Despite non-pathogenicity, the existence of multiple viruses within a bird species underscores the risk of these viruses spreading into the pet trade. Detection and a better understanding of avian viruses are crucial for the establishment of appropriate management and biosecurity measures in the domestic and international bird trade, which ultimately supports the conservation of vulnerable bird species.

Item ID:	83904
Item Type:	Article (Research - C1)
ISSN:	2053-1095
Keywords:	adenovirus,beak and feather disease virus,chaphamaparvovirus,metagenomics,next-generation sequencing,parrot
Related URLs:	https://www.scopus.com/inward/record.uri...
Copyright Information:	© 2024 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Funders:	Australian Research Council (ARC)
Projects and Grants:	ARC Discovery Early Career Researcher Award (grant number DE200100367)
Date Deposited:	28 Oct 2024 23:48
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