Model for the controlled synthesis of O-antigen repeat units involving the WaaL ligase
Hong, Yaoqin, and Reeves, Peter R. (2016) Model for the controlled synthesis of O-antigen repeat units involving the WaaL ligase. mSphere, 1 (1).
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Abstract
The Wzx/Wzy O-antigen pathway involves synthesis of a repeat unit (O unit) consisting of 3 to 8 sugars on an inner-membrane-embedded lipid carrier. These O units are translocated across the membrane to its periplasmic face by Wzx, while retaining linkage to the carrier, and then polymerized by Wzy to O-antigen polymer, which WaaL ligase transfers to a lipopolysaccharide precursor to complete lipopolysaccharide synthesis, concomitantly releasing the lipid carrier. This lipid carrier is also used for peptidoglycan assembly, and sequestration is known to be toxic. Thus, O-unit synthesis must involve precise regulation to meet demand but avoid overproduction. Here we show that loss of WaaL reverses a known growth defect in a Salmonella mutant that otherwise accumulates O-unit intermediates and propose that WaaL is also involved in a novel feedback mechanism to regulate O-unit synthesis, based on the availability of O units on the periplasmic face of the membrane.
| Item ID: | 83818 |
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| Item Type: | Article (Research - C1) |
| ISSN: | 2379-5042 |
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| Copyright Information: | Copyright © 2015 Hong and Reeves. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
| Funders: | Australian Research Council (ARC) |
| Projects and Grants: | ARC DP140104068 |
| Date Deposited: | 22 Oct 2024 01:47 |
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