Karnaneedi, Shaymaviswanathan, Johnston, Elecia B., Bose, Utpal, Juhász, Angéla, Broadbent, James A., Ruethers, Thimo, Jerry, Emily M., Kamath, Sandip D., Limviphuvadh, Vachiranee, Stockwell, Sally, Byrne, Keren, Clarke, Dean, Colgrave, Michelle L., Maurer-Stroh, Sebastian, and Lopata, Andreas L. (2024) The Allergen Profile of Two Edible Insect Species—Acheta domesticus and Hermetia illucens. Molecular Nutrition & Food Research, 68 (16). 2300811.

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Abstract

Scope: Edible insect proteins are increasingly introduced as an alternative sustainable food source to address the world's need to feed the growing population. Tropomyosin is the main insect allergen; however, additional potential allergens are not well characterized and the impact of extraction procedures on immunological reactivity is unknown.

Methods and results: Proteins from different commercial food products derived from cricket (Acheta domesticus) and black soldier fly (BSF) (Hermetia illucens) are extracted using five different extraction buffers. The proteins are analyzed by SDS-PAGE and immunoblotting using allergen-specific antibodies and crustacean allergic patient sera. IgE binding bands are analyzed by mass spectrometry as well as the complete allergen profile of all 30 extracts. Urea-based buffers are most efficient in extracting insect allergens. Shrimp-specific antibody cross-reactivity to tropomyosin from cricket and BSF indicates high sequence and structural similarity between shrimp and insects. Additional unique allergens are identified in both species, including hemocyanin, vitellogenin, HSP20, apolipophorin-III, and chitin-binding protein.

Conclusions: Identifying potential allergenic proteins and their isoforms in cricket and BSF requires specific extraction approaches using urea-based methods. While tropomyosin is the most abundant and immunoreactive allergen, seven unique allergens are identified, highlighting the need for insect species-specific allergen detection in food products.

Item ID:	83684
Item Type:	Article (Research - C1)
ISSN:	1613-4133
Keywords:	allergen,black soldier fly,cricket,edible insect,food allergy
Related URLs:	https://www.scopus.com/inward/record.uri...
Copyright Information:	©2024 The Author(s). Molecular Nutrition & Food Research published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Funders:	National Health and Medical Research Council (NHMRC)
Projects and Grants:	NHMRC Grant number: GNT1086656
Date Deposited:	24 Sep 2024 01:47
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