Dorney, Ryley D., Johnston, Elecia B., Karnaneedi, Shaymaviswanathan, Ruethers, Thimo, Kamath, Sandip D., Gopi, Karthik, Mazumder, Debashish, Sammut, Jesmond, Jerry, Dean, Williamson, Nicholas A., Nie, Shuai, and Lopata, Andreas L. (2024) Variation in Shrimp Allergens: Place of Origin Effects on Food Safety Assessment. International Journal of Molecular Sciences, 25. 4531.

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Abstract

Due to the widespread use of shellfish ingredients in food products, accurate food labelling is urgently needed for consumers with shellfish allergies. Most crustacean allergen detection systems target the immunorecognition of the allergenic protein tropomyosin. However, this mode of detection may be affected by an origin-dependent protein composition. This study determined if the geographic location of capture, or aquaculture, influenced the allergenic protein profiles of Black Tiger Shrimp (Penaeus monodon), one of the most farmed and consumed shrimp species worldwide. Protein composition was analysed in shrimp from nine different locations in the Asia-Pacific by SDS-PAGE, immunoblotting, and mass spectrometry. Ten of the twelve known shrimp allergens were detected, but with considerable differences between locations. Sarcoplasmic calcium-binding protein, myosin light chain, and tropomyosin were the most abundant allergens in all locations. Hemocyanin-specific antibodies could identify up to six different isoforms, depending on the location of origin. Similarly, tropomyosin abundance varied by up to 13 times between locations. These findings suggest that allergen abundance may be related to shrimp origin and, thus, shrimp origin might directly impact the readout of commercial crustacean allergen detection kits, most of which target tropomyosin, and this should be considered in food safety assessments.

Item ID:	83680
Item Type:	Article (Research - C1)
ISSN:	1422-0067
Keywords:	allergen ELISA,crustacean allergens,food allergen proteomics,mass spectrometric analysis of allergens, shellfish, tropomyosin
Related URLs:	https://www.scopus.com/inward/record.uri...
Copyright Information:	© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Date Deposited:	19 Sep 2024 04:00
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