Laklaeng, Sa-ngob, Songsri, Jirarat, Wisessombat, Sueptrakool, Mala, Wanida, Phothaworn, Preeda, Senghoi, Wilaiwan, Nuinoon, Manit, Tangphatsornruang, Sithichoke, Wongtawan, Teumpong, Hayakijkosol, Orachun, Kerdsin, Anusak, and Kwanhian Klangbud, Wiyada (2024) Multi-locus sequence typing and genetic diversity of antibiotic-resistant genes and virulence-associated genes in Burkholderia pseudomallei: Insights from whole genome sequencing of animal and environmental isolates in Thailand. Veterinary Microbiology, 298. 110236.

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Abstract

Burkholderia pseudomallei is a Gram-negative bacillus and the etiological agent of melioidosis in humans and animals. The disease is highly endemic in northern Australia and Southeast Asia. Comprehensive genomic data are essential for understanding the bacteria’s dissemination and genetic relationships among strains from different geographical regions. In this study, we conducted antimicrobial susceptibility testing and wholegenome sequencing of 54 B. pseudomallei isolates obtained from environmental and animal sources in southern Thailand between 2011 and 2018. Their genomics were determined of antibiotic-resistant genes (ARGs), virulence-associated genes, mobile genetic elements (MGEs), sequence types (STs), and single nucleotide polymorphisms (SNPs) to evaluate their epidemiological relatedness. Remarkably, all 54 isolates displayed sensitivity to antimicrobial agents typically used for melioidosis treatment. We identified nine distinct sequence types: ST392, ST51, ST409, ST508, ST376, ST1721, ST389, ST395, and ST289. Oxacillinase genes and the resistance nodulation family of efflux pumps (RND) were identified as contributors to antimicrobial resistance. Phylogenetic analysis demonstrated close genetic relations with other strains isolated from Southeast Asia. Furthermore, 172 virulence-associated genes were identified among the isolates, suggesting variations in clinical presentations. These findings underscore the importance of ongoing molecular genetic surveillance of B. pseudomallei for effective healthcare management and reducing melioidosis mortality.

Item ID:	83572
Item Type:	Article (Research - C1)
ISSN:	1873-2542
Copyright Information:	© 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
Date Deposited:	10 Sep 2024 01:28
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300911 Veterinary pharmacology @ 50% 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300904 Veterinary diagnosis and diagnostics @ 50%
SEO Codes:	20 HEALTH > 2099 Other health > 209999 Other health not elsewhere classified @ 80% 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1001 Environmentally sustainable animal production > 100199 Environmentally sustainable animal production not elsewhere classified @ 20%
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