Wong, Yide, Pearson, Mark S., Fedorova, Olga, Ivanov, Vladimir, Khmelevskaya, Ekaterina, Tedla, Bemnet, Arachchige, Buddhika Jayakody, Reed, Sarah, Field, Matt, Laha, Thewarach, Loukas, Alex, and Sotillo-Gallego, Javier (2023) Secreted and surface proteome and transcriptome of Opisthorchis felineus. Frontiers in Parasitology, 2. 1195457.

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Abstract

Introduction: Opisthorchis felineus, Opisthorchis viverrini, and Clonorchis sinensis are the most medically important species of fish-borne zoonotic trematodes. O. felineus is endemic to the river plains of Western Siberia and Eastern Europe, and it is estimated that more than 1.6 million people could be infected with this parasite. Chronic opisthorchiasis may lead to significant gastrointestinal and hepatobiliary pathology. This study aimed to identify and characterize proteins from the secreted and tegumental proteomes of O. felineus.

Methods: Adult flukes were collected from experimentally infected hamsters and cultured in vitro in serum-free media. We extracted proteins from different compartments of the O. felineus secretome, including (i) soluble excretory/secretory (ES) products; (ii) secreted 15K-extracellular vesicles (EVs); and (iii) tegument.

Results: We also generated a transcriptome using long-read sequencing, and when this was combined with high-resolution mass spectrometry, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) separation, and protein digestion, we identified 686, 894, 389, 324, and 165 proteins from the ES, 15K-EV, and the three sequentially extracted tegument (TEG) protein fractions, respectively. We conducted in-depth gene ontology and protein family analyses on the identified proteins and discussed comparisons against similar proteome data sets acquired for the Southeast Asian liver fluke O. viverrini and the Chinese liver fluke C. sinensis.

Discussion: The information from this study will form a biologically relevant data set of O. felineus proteins that could be used to develop diagnostic and therapeutic tools to manage the human cost of O. felineus infection and its associated comorbidities.

Item ID:	83483
Item Type:	Article (Research - C1)
ISSN:	2813-2424
Related URLs:	http://dx.doi.org/10.3389/fpara.2023.119...
Copyright Information:	Copyright © 2023 Wong, Pearson, Fedorova, Ivanov, Khmelevskaya, Tedla, Arachchige, Reed, Field, Laha, Loukas and Sotillo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Funders:	National Health and Medical Research Council (NHMRC)
Projects and Grants:	NHMRC e-ASIA Joint Research Program APP1185434, NHMRC Investigator Level Three Grant APP2008450
Date Deposited:	26 Aug 2024 22:43
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