Mihaila, Alisa A., Lawton, Rebecca J., Glasson, Christopher R.K., and Magnusson, Marie (2023) Early hatchery protocols for tetrasporogenesis of the antimethanogenic seaweed Asparagopsis armata. Journal of Applied Phycology, 35. pp. 2323-2335.

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Abstract

The red alga Asparagopsis armata is an emerging aquaculture-target species due to its application as an antimethanogenic feed ingredient in ruminants, yet information on A. armata reproduction and cultivation is currently lacking. We therefore quantified the effects of temperature, irradiance, nutrients, and photoperiod, and addition of plant growth regulators (PGRs; indole-3-acetic acid, abscisic acid, 1-aminocyclopropane-1-carboxylic acid) on tetrasporogenesis in domesticated A. armata that had been maintained under controlled conditions (18 °C, 12 h light:12 h dark photoperiod) for 18 months prior to experimentation. Tetrasporogenesis was only induced at 5 and 15 µmol photons m−2 s−1 under an 8 h light:16 h dark photoperiod with 3.5 mg nitrogen L−1 and tetraspore release was 28-fold greater at 18 °C compared to 15 °C after 28 days of exposure. After 29 days, tetraspore release and germination rate both declined. All PGR treatments prevented tetrasporogenesis. This study is the first to provide the detail and framework necessary to enable A. armata hatchery development. We conclude that tetrasporogenesis was most likely induced in response to a significant reduction in photoperiod rather than as a result of replicating seasonal environmental conditions, and that temperature played a key role in determining reproductive output. With overall higher tetraspore release and a consistent germination rate of > 90%, we recommend exposing tetrasporophyte biomass to 18 °C, 15 µmol photons m−2 s−1 and 3.5 mg nitrogen L−1 under an 8 h L:16 h D photoperiod for up to 29 days to obtain a reliable supply of tetraspores for seeding onto ropes for transfer to the hatchery phase.

Item ID:	83335
Item Type:	Article (Research - C1)
ISSN:	1573-5176
Copyright Information:	© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Date Deposited:	05 Aug 2024 23:47
FoR Codes:	31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310801 Phycology (incl. marine grasses) @ 100%
SEO Codes:	26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2606 Industrial crops > 260699 Industrial crops not elsewhere classified @ 50% 26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2699 Other plant production and plant primary products > 269999 Other plant production and plant primary products not elsewhere classified @ 50%
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