Thye, Kah-Lok, Abdullah, Wan Muhamad Asrul Nizam Wan, Ong-Abdullah, Janna, Lamasudin, Dhilia Udie, Wee, Chien-Yeong, Mohd Yusoff, Mohd Hafis Yuswan, Loh, Jiun-Yan, Cheng, Wan-Hee, and Lai, Kok-Song (2023) Calcium lignosulfonate modulates physiological and biochemical responses to enhance shoot multiplication in Vanilla planifolia Andrews. Physiology and Molecular Biology of Plants, 29. pp. 377-392.

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Abstract

Utilisation of calcium lignosulfonate (CaLS) in Vanilla planifolia has been reported to improve shoot multiplication. However, mechanisms responsible for such observation remain unknown. Here, we elucidated the underlying mechanisms of CaLS in promoting shoot multiplication of V. planifolia via comparative proteomics, biochemical assays, and nutrient analysis. The proteome profile of CaLS-treated plants showed enhancement of several important cellular metabolisms such as photosynthesis, protein synthesis, Krebs cycle, glycolysis, gluconeogenesis, and carbohydrate synthesis. Further biochemical analysis recorded that CaLS increased Rubisco activity, hexokinase activity, isocitrate dehydrogenase activity, total carbohydrate content, glutamate synthase activity and total protein content in plant shoot, suggesting the role of CaLS in enhancing shoot growth via upregulation of cellular metabolism. Subsequent nutrient analysis showed that CaLS treatment elevated the contents of several nutrient ions especially calcium and sodium ions. In addition, our study also revealed that CaLS successfully maintained the cellular homeostasis level through the regulation of signalling molecules such as reactive oxygen species and calcium ions. These results demonstrated that the CaLS treatment can enhance shoot multiplication in V. planifolia Andrews by stimulating nutrient uptake, inducing cell metabolism, and regulating cell homeostasis.

Item ID:	83260
Item Type:	Article (Research - C1)
ISSN:	0974-0430
Copyright Information:	© Prof. H.S. Srivastava Foundation for Science and Society 2023
Date Deposited:	30 Jul 2024 02:12
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3001 Agricultural biotechnology > 300101 Agricultural biotechnology diagnostics (incl. biosensors) @ 100%
SEO Codes:	26 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 2606 Industrial crops > 260699 Industrial crops not elsewhere classified @ 100%
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