Gal, Arnon, Barko, Patrick C., Biggs, Patrick J., Gedeye, Kristene R., Midwinter, Anne C., Williams, David A., Burchell, Richard K., and Pazzi, Paolo (2021) One dog’s waste is another dog’s wealth: A pilot study of fecal microbiota transplantation in dogs with acute hemorrhagic diarrhea syndrome. PLoS ONE, 16 (4). e0250344.

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Abstract

Canine acute hemorrhagic diarrhea syndrome (AHDS) hasbeen associated insome studies with Clostridioides perfringens overgrowth and toxin-mediated necrosis of the intestinal mucosa. Weaimedtodetermine the effect of a single fecal microbiota transplantation (FMT) onclinical scores and fecal microbiomes of 1 and 7 dogs with AHDS from New Zealand and South Africa. We hypothesized that FMT would improve AHDS clinical scores and increase microbiota alpha-diversity and short-chain fatty acid (SCFA)-producing microbial communities’ abundances in dogs with AHDS after FMT. Wesequenced theV3-V4 region of the 16S-rRNA genein the feces of AHDSFMT-recipients andsham-treated control dogs, andtheir healthy donors at admission, discharge, and 30 days post-discharge. There were no significant differences in median AHDS clinical scores between FMT-recipients and sham-treated controls at admission or discharge (P = 0.22, P = 0.41). At admission, the Shannon diversity index (SDI) was lower in AHDS dogs than healthy donors (P = 0.002). TheSDIdidnotchange from admission to 30daysin sham-treated dogs yet increased in FMT-recipients from admission to discharge (P = 0.04) to levels not different than donors (P =0.33) but significantly higher than sham-treated controls (P = 0.002). At 30 days, the SDI did not differ between FMT recipients, sham-treated controls, and donors (P = 0.88). Principal coordinate analysis of the Bray-Curtis index separated post-FMT and donor dogs from pre-FMT andsham-treated dogs (P =0.009) because of increased SCFA-producing genera’s abundances after FMT. A single co-abundance subnetwork contained many of the sameOTUsfoundtobedifferentially abundant in FMT-recipients, and the abundance of this module was increased in FMT-recipients at discharge and 30 days, compared to shamtreated controls. We conclude in this small pilot study FMT did not have any clinical benefit.

Item ID:	83016
Item Type:	Article (Research - C1)
ISSN:	1932-6203
Copyright Information:	© 2021 Gal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Date Deposited:	24 Jun 2024 23:11
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300907 Veterinary medicine (excl. urology) @ 100%
SEO Codes:	20 HEALTH > 2099 Other health > 209999 Other health not elsewhere classified @ 100%
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