Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Nkouawa, Agathe, Okamoto, Munehiro, Mabou, Alida Kouojip, Edinga, Eulodie, Yamasaki, Hiroshi, Sako, Yasuhito, Nakao, Minoru, Nakaya, Kazuhiro, Blair, David, Agatsuma, Takeshi, Enyong, Peter, Shibahara, Toshiyuki, Moyou-Somo, Roger, and Ito, Akira (2009) Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations. Transactions of the Royal Society of Tropical Medicine and Hygiene, 103 (3). pp. 255-261.

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Abstract

Paragonimiasis is a common parasitic zoonosis in Cameroon and neighbouring countries in Western Africa. Serum, sputum and faecal samples were collected in an endemic area of South West Province, Cameroon, after administration of a questionnaire to identify individuals with appropriate symptoms and histories. Microscopic examination revealed eggs in sputum from 16 people, but none in any faecal sample. These 16 were among the 25 and 26 people, respectively, positive by ELISA and by immunoblot using Paragonimus africanus crude antigens. Copro-DNA detection was attempted using 23 faecal samples (18 from sputum egg-negative and five from sputum egg-positive individuals). Copro-DNA was detected in four of the five sputum egg-positive individuals. These results strongly suggest that: (1) serology is much more sensitive than sputum examination for diagnosis of paragonimiasis; and (2) a copro-DNA test may be more sensitive than a microscopic search for eggs in faeces. Molecular sequence data from ITS2 and cox1 genes confirmed that adult worms experimentally raised in cats were P. africanus and that eggs from sputum or other worm products from human faeces also belonged to this species. Based on these results, 26 of 168 persons (15.5%) were diagnosed as suffering from paragonimiasis.

Item ID: 7966
Item Type: Article (Refereed Research - C1)
Keywords: paragonimiasis; Paragonimus africanus; diagnosis; serology; copro-DNA; Cameroon; tropical biology
ISSN: 0035-9203
Date Deposited: 20 Jan 2010 05:47
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 60%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 40%
SEO Codes: 92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920404 Disease Distribution and Transmission (incl. Surveillance and Response) @ 50%
92 HEALTH > 9202 Health and Support Services > 920203 Diagnostic Methods @ 50%
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